Gene mutations can lead to altered gene function and malignant cellular transformation. Therefore, to improve the early detection of mutations, it is indispensable to have a complete map of cancer driver genes and their hot spots. Thus, the goal of the our research target 1 is to confirm hot spot mutations in Cancer driver genes using Crispr-Cas9 technology. Moreover, we will also establish a novel screening system to identify cancer driver genes using a gene mutation method in vivo. Since the development of the Next Generation Sequencer, there has been an ever increasing number of research projects investigating the cancer genome. These studies have identified many single nucleotide variants and copy number alterations. For example, the International Cancer Genome Consortium has coordinated a large number of research projects that have the common aim of comprehensively elucidating the genomic changes present in many forms of cancers. Though quite useful, the results of these high-throughput research projects must be validated through functional studies. Thus, we plan to perform a functional analysis on the several genes of transcription factors using the novel approach. Our expertise in the field of cancer biology and focus on transcriptional gene regulation will ensure that this project is well positioned to make exciting new contributions to this field of study. Undoubtedly, the findings of our study will support the ongoing efforts aimed at improving the early detection of cancers, while also contributing to the field of preventive medicine.